Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 2. mRNA expression levels of VEGF and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Expressing, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 3. Immunohistochemical analysis of VEGF expression in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Immunohistochemistry staining of VEGF in the articular cartilage of the medial tibial plateau (magnification, x400, scale bar=100 µm). (B) Quantification of VEGF positive cells, based on the results of immunohistochemistry staining. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Immunohistochemical staining, Expressing, Immunohistochemistry, Staining, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 5. ELISA analysis of serum VEGF concentration of mice among the Sham, Dmm and Dmm+Th groups (n=8 in each group). The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation

Journal: bioRxiv

Article Title: Immunoregulatory subtype of dermal lymphatic endothelial cells at capillary terminals drives lymphatic malformations

doi: 10.1101/2022.05.22.492950

Figure Lengend Snippet: ( A-E ) Quantification of the CD45 + area ( A , B ) and F4/80 + area ( C - E ) in the ear skin showing increase in Pik3ca H1047R ;Vegfr3-CreER T2 ( A, C, D ) but not in Pik3ca H1047R ;Vegfr1-CreER T2 ( B, C, E ) mice. Data represent mean (CD45: n =4-6 images from n =3-5 mice per genotype; F4/80: n =3-7 images from n =2-3 mice per genotype) ± s.e.m. Representative binary images are shown below the graphs. ( F, G ) Flow cytometry analysis of innate ( F ) and adaptive ( G ) immune cells in the ear skin of 4-OHT-treated 5-week-old and 10-week-old Pik3ca H1047R ; Vegfr3-CreER T2 mice and littermate controls. Mac, macrophage; Mono, monocyte; DC, dendritic cell; Neu, neutrophil; NK, natural killer cell. Data represent relative cell frequency (of live cells) relative to the control ( n =3-9 mice) ± s.e.m. ( H ) Multiplex ELISA analysis of pro-inflammatory cytokines and chemokines associated with recruitment and/or activation of myeloid cells in whole ear skin lysates from Pik3ca H1047R ;Vegfr3-CreER T2 mice and littermate controls. Data represent mean protein levels relative to control ( n =6-11 mice) ± s.e.m. ( I ) qRT-PCR analysis (left) and ELISA analysis (right) of Vegfc /VEGF-C levels in the ear skin lysates of 4-OHT-treated 5-week-old Pik3ca H1047R ;Vegfr3-CreER T2 and littermate control mice. qRT-PCR data represent mean relative expression (normalized to Hprt ; n =7-10 mice) ± s.e.m. Transcript and protein levels are presented relative to controls. ( J ) qRT-PCR analysis of Vegfc in CD45 + Cd11b + F4/80 + macrophages and CD45 + Cd11b + F4/80 + Ly6C + monocytes, FACS-sorted from the ear skin of 4-OHT-treated 5-week-old Pik3ca H1047R ;Vegfr3-CreER T2 and littermate control mice. qRT-PCR data represent mean relative expression (normalized to Hprt ; n =3-5 mice) ± s.e.m. Transcript and protein levels are presented relative to macrophages/monocytes in control mice. p -value in (A, B, D-I) obtained using Two-tailed unpaired Student’s t-test. Scale bar: 100 μm (A-E).

Article Snippet: Mouse Vascular Endothelial Cell Growth Factor C (VEGF-C) ELISA Kit from CUSABIO (CSB-E07361m) was used for detection of VEGF-C protein concentration.

Techniques: Flow Cytometry, Control, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Activation Assay, Quantitative RT-PCR, Expressing, Two Tailed Test

Journal: bioRxiv

Article Title: Immunoregulatory subtype of dermal lymphatic endothelial cells at capillary terminals drives lymphatic malformations

doi: 10.1101/2022.05.22.492950

Figure Lengend Snippet: ( A ) Flow cytometry analysis of the number of CD45 + CD11b + F4/80 + macrophages in the ear skin of 4-OHT-treated 5-week-old Pik3ca H1047R ;Vegfr3-CreER T2 ( n =5) and control ( n =3) mice. Data represent mean cell number per gram tissue ± s.e.m. p -value, Mann-Whitney U Test. ( B ) Flow cytometry analysis of the frequency of CD45 + CD11b + F4/80 + antigen-presenting myeloid cells in the ear skin of 4-OHT-treated 5-week-old Pik3ca H1047R ;Vegfr1-CreER T2 ( n =6) and control ( n =5) mice. Data represent relative cell frequency (of live cells) relative to the control ± s.e.m. p -value obtained using Two-tailed unpaired Student’s t-test. ( C ) Flow cytometry analysis of innate (left) and adaptive (right) immune cells in the ear skin of 4-OHT-treated 10-week-old Pik3ca H1047R ; Vegfr1-CreER T2 mice and littermate controls. Mac, macrophage; Mono, monocyte; DC, dendritic cell; Neu, neutrophil; NK, natural killer cell. Data represent relative cell frequency (of live cells) relative to the control ( n =5-8 mice for innate panel, n=7-9 mice for adaptive panel) ± s.e.m. p -value obtained using Two-tailed unpaired Student’s t-test. ( D, E ) Multiplex ELISA analysis of pro-inflammatory cytokines and chemokines associated with recruitment and/or activation of myeloid cells or T-cells and B-cells in whole ear skin lysates from Pik3ca H1047R ;Vegfr1-CreER T2 ( D ) and Pik3ca H1047R ;Vegfr3-CreER T2 ( E ) mice, and respective littermate controls. ( F ) Similar analysis of TNFα and INFγ in blood serum of Pik3ca H1047R ;Vegfr3-CreER T2 mice. Data in ( D-E ) represent mean protein levels ( n =3-9 mice) ± s.e.m.

Article Snippet: Mouse Vascular Endothelial Cell Growth Factor C (VEGF-C) ELISA Kit from CUSABIO (CSB-E07361m) was used for detection of VEGF-C protein concentration.

Techniques: Flow Cytometry, Control, MANN-WHITNEY, Two Tailed Test, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Activation Assay